What Is Testosterone Levels?
Testosterone is the primary male sex hormone (androgen), but it is also crucial for women's health. A testosterone levels test measures the amount of this hormone circulating in your blood. The test typically quantifies total testosterone, which includes both protein-bound and free hormone, and sometimes free testosterone specifically, which is the biologically active form not bound to proteins like sex hormone-binding globulin (SHBG).
Doctors order this test to diagnose and manage a variety of conditions. For men, it's commonly used to evaluate symptoms of hypogonadism (low testosterone), such as low libido, erectile dysfunction, fatigue, and loss of muscle mass. It also helps assess delayed or early puberty in boys, and investigate causes of infertility. For women, testing is used to diagnose conditions like polycystic ovary syndrome (PCOS), which can cause elevated testosterone, leading to irregular periods, acne, and hirsutism. It can also help evaluate virilization (development of male characteristics) and explore causes of low libido or menopausal symptoms.
Testosterone production relates primarily to two organ systems. In men, about 95% is produced by the Leydig cells in the testes, stimulated by luteinizing hormone (LH) from the pituitary gland. In women, the ovaries and adrenal glands produce smaller amounts. The hypothalamus-pituitary-gonadal (HPG) axis tightly regulates its production: the hypothalamus releases gonadotropin-releasing hormone (GnRH), which signals the pituitary to release LH, which in turn stimulates testosterone production. Therefore, abnormal levels can indicate a problem with the testes/ovaries (primary issue) or with the pituitary/hypothalamus (secondary issue).
Normal Testosterone Levels Ranges
By Age and Gender
Reference ranges can vary between laboratories, but the following table provides evidence-based ranges from major institutions like the Endocrine Society and Mayo Clinic Laboratories. The "Optimal" column reflects functional medicine ranges often associated with peak well-being and preventive health, which may be narrower than standard reference intervals.
| Group | Low | Normal (Reference Range) | Optimal (Functional Range) | High |
|---|---|---|---|---|
| Adult Men (19-39 yrs) | < 264 ng/dL < 9.2 nmol/L | 264 - 916 ng/dL 9.2 - 31.8 nmol/L | 500 - 800 ng/dL 17.3 - 27.7 nmol/L | > 916 ng/dL > 31.8 nmol/L |
| Adult Women (19-39 yrs) | < 15 ng/dL < 0.5 nmol/L | 15 - 70 ng/dL 0.5 - 2.4 nmol/L | 25 - 50 ng/dL 0.9 - 1.7 nmol/L | > 70 ng/dL > 2.4 nmol/L |
| Children (Pre-puberty) | — | < 10 ng/dL < 0.35 nmol/L | — | > 10 ng/dL > 0.35 nmol/L |
| Pregnant Women | — | Levels can rise to 3-4x normal | Not applicable | — |
| Elderly Men (70+ yrs) | < 212 ng/dL < 7.4 nmol/L | 212 - 742 ng/dL 7.4 - 25.7 nmol/L | 350 - 600 ng/dL 12.1 - 20.8 nmol/L | > 742 ng/dL > 25.7 nmol/L |
What "Normal" vs "Optimal" Means
The "Normal" or reference range is a statistical concept derived from testing a large, apparently healthy population. It typically encompasses the middle 95% of results, meaning 5% of healthy people will naturally fall outside it. A result within this range is considered statistically normal for the population but may not be optimal for an individual's health, energy, or vitality.
"Optimal" ranges, often used in functional and preventive medicine, represent levels associated with the lowest risk of disease and the highest reported sense of well-being, libido, and physical function. For example, a man with a testosterone level of 280 ng/dL is within the standard normal range but may experience symptoms of low T and have a higher metabolic risk. According to research in journals like The Journal of Clinical Endocrinology & Metabolism, levels in the lower third of the normal range are associated with increased risk of metabolic syndrome, cardiovascular disease, and mortality. Therefore, aiming for an optimal range matters for preventive health, as it supports long-term metabolic, cognitive, and musculoskeletal health beyond just avoiding clinical deficiency.
What Do Abnormal Results Mean?
High Testosterone Levels
Causes:- Polycystic Ovary Syndrome (PCOS): The most common cause in women, due to hormonal imbalance and insulin resistance.
- Congenital Adrenal Hyperplasia: A genetic disorder causing overproduction of androgens from the adrenal glands.
- Tumors: Rare androgen-producing tumors of the ovary, testicle, or adrenal gland.
- Anabolic Steroid or Androgen Use: Exogenous testosterone or performance-enhancing drugs.
- Hyperthyroidism: Can increase sex hormone-binding globulin (SHBG) and alter hormone balance.
- Acromegaly: Excess growth hormone can disrupt the HPG axis.
- Testosterone Resistance: Rare condition where the body doesn't respond to testosterone, leading to high levels due to feedback loops.
Low Testosterone Levels
Causes:- Primary Hypogonadism (Testicular Failure): Causes include Klinefelter syndrome, undescended testes, mumps orchitis, chemotherapy/radiation, or physical injury.
- Secondary Hypogonadism (Pituitary/Hypothalamus Issue): Causes include pituitary disorders, hyperprolactinemia, chronic opioid use, obesity, metabolic syndrome, type 2 diabetes, and excessive alcohol use.
- Aging: Gradual decline is normal (andropause), but significant symptomatic decline is not inevitable.
- Chronic Illness: Kidney/liver disease, HIV/AIDS, and chronic inflammatory conditions.
- Sleep Apnea: Severe obstructive sleep apnea is strongly linked to low testosterone.
- Severe Stress & Cortisol Imbalance: Chronically high cortisol can suppress the HPG axis.
- Medications: Glucocorticoids, opioids, and some hormones (like anabolic steroid abuse followed by withdrawal).
How to Improve Your Testosterone Levels
Diet and Nutrition
A balanced, nutrient-dense diet is foundational. Specific foods can support healthy testosterone production by providing essential fats, minerals (like zinc and magnesium), and vitamins.
Foods to Emphasize:- Healthy Fats: Monounsaturated and saturated fats from sources like avocados, olive oil, nuts, and eggs are precursors for hormone synthesis.
- Lean Protein: Adequate protein (e.g., grass-fed beef, chicken, fish) supports muscle mass and can aid in weight management, a key factor for testosterone.
- Zinc-Rich Foods: Oysters are exceptionally high in zinc. Other sources include beef, pumpkin seeds, and lentils. Zinc deficiency is directly linked to low testosterone.
- Vitamin D Sources: Fatty fish (salmon, mackerel), egg yolks, and fortified foods. Vitamin D acts like a hormone and is strongly correlated with testosterone levels.
- Excessive Alcohol: Chronic heavy consumption can damage Leydig cells and increase estrogen conversion.
- Highly Processed Foods & Sugars: Contribute to obesity and insulin resistance, which lower testosterone.
- Soy Products (in very high amounts): Contain phytoestrogens, which may have a mild estrogenic effect in some individuals when consumed in extreme quantities.
- Trans Fats: Found in fried and packaged foods, they are linked to reduced testosterone and poor overall health.
| Food | Key Nutrient(s) | Suggested Serving |
|---|---|---|
| Oysters | Zinc | 6 medium oysters (50mg zinc) |
| Grass-fed Beef | Zinc, Protein, Saturated Fat | 3-4 oz cooked |
| Tuna/Salmon | Vitamin D, Protein, Omega-3s | 3-4 oz cooked |
| Egg Yolks | Vitamin D, Cholesterol, Saturated Fat | 2-3 whole eggs |
| Pumpkin Seeds | Zinc, Magnesium | 1/4 cup |
| Spinach | Magnesium | 1 cup cooked |
| Pomegranate | Antioxidants (may boost testosterone) | 1 cup arils or 8 oz juice |
| Garlic | Allicin (may reduce cortisol) | 2-3 cloves |
| Brazil Nuts | Selenium | 2-3 nuts |
| Extra Virgin Olive Oil | Monounsaturated Fats | 1-2 Tbsp |
Lifestyle Changes
Exercise:- Resistance Training: Heavy compound lifts (squats, deadlifts, bench presses) are most effective. Aim for 3-4 sessions per week, focusing on progressive overload.
- High-Intensity Interval Training (HIIT): Can boost growth hormone and testosterone short-term. Avoid excessive chronic cardio, which may elevate cortisol and lower T.
- Stress Management: Chronic stress elevates cortisol, which directly antagonizes testosterone production. Incorporate mindfulness, meditation, or deep breathing exercises.
- Vitamin D3: 2,000 - 5,000 IU daily. Correcting a deficiency is one of the most effective interventions. (Strong Evidence)
- Zinc: 25 - 40 mg daily (often as zinc picolinate). Especially beneficial if deficient. (Moderate Evidence)
- Magnesium: 200 - 400 mg daily (as glycinate or citrate). Supports hundreds of enzymatic processes, including hormone production. (Moderate Evidence)
- DHEA: A precursor hormone. Use only under medical supervision, as it can convert to both androgens and estrogens. Dosage is highly individualized. (Evidence for deficiency states)
Related Tests to Consider
Testosterone does not act in isolation. Testing these related biomarkers provides a fuller picture of your hormonal health:
- Free Testosterone: Measures the active, unbound fraction. Crucial if total T is borderline or SHBG is abnormal.
- Sex Hormone-Binding Globulin (SHBG): Binds to testosterone. High SHBG lowers free T; low SHBG (common in obesity) can elevate free T.
- Luteinizing Hormone (LH): Distinguishes primary (high LH) from secondary (low/normal LH) hypogonadism.
- Estradiol (E2): The primary estrogen. Testosterone can convert to estradiol via aromatase. Balancing T and E2 is key for both men and women.
- Prolactin: Markedly elevated prolactin can suppress testosterone production.
- Comprehensive Metabolic Panel (CMP) & Lipid Panel: To assess metabolic health, liver/kidney function, and cardiovascular risk, all interconnected with hormone status.
When to Get Tested
Recommended Testing Frequency: For asymptomatic adults, it's not part of routine screening. Testing is symptom-driven. If on testosterone replacement therapy (TRT), monitoring every 3-6 months initially, then every 6-12 months is standard. Risk Factors Warranting Testing:- Men: Symptoms of low T, infertility, osteoporosis, type 2 diabetes, metabolic syndrome, HIV/AIDS, or history of chemotherapy/radiation.
- Women: Symptoms of PCOS (hirsutism, irregular periods), virilization, or suspected adrenal disorders.
- Timing: For men, blood draw should be between 7 AM and 10 AM when levels peak. For women, timing may relate to menstrual cycle (often early follicular phase).
- Fasting: Not always required, but some doctors recommend it as a lipid panel is often drawn concurrently.
- Other: Get adequate sleep the night before. Inform your doctor of all medications/supplements. Avoid biotin supplements (>5mg/day) for 72 hours prior, as they can interfere with some lab assays.
Frequently Asked Questions
1. Is a testosterone level of 350 ng/dL normal for a 45-year-old man?Yes, it falls within the standard normal reference range for adult men. However, it is in the lower third, and many men with this level may experience symptoms like fatigue and low libido. It may be considered suboptimal for preventive health.
2. What is a dangerously high testosterone level?In men, levels persistently above 1200 ng/dL, especially if caused by exogenous steroid use, pose risks like polycythemia (high red blood cell count), severe acne, liver strain, and cardiac issues. In women, levels above 70-80 ng/dL typically indicate an underlying condition like PCOS or a tumor that requires investigation.
3. Can stress really lower testosterone?Yes. Chronic stress elevates cortisol, which directly suppresses the hypothalamic-pituitary-gonadal (HPG) axis, reducing the signals that tell your testes to produce testosterone.
4. Do testosterone boosters from the store work?Most over-the-counter "boosters" contain vitamins and herbs (like zinc, D3, ashwagandha). They may help if you have a deficiency, but they are unlikely to raise levels significantly if you are already in a normal range. They are not a substitute for prescribed therapy in cases of true hypogonadism.
5. What level of testosterone requires TRT (Testosterone Replacement Therapy)?The Endocrine Society guidelines suggest considering TRT for symptomatic men with consistently low total testosterone levels below 300 ng/dL. The decision heavily depends on the severity of symptoms, patient age, and other health factors.
6. How can I raise my testosterone naturally?Focus on the pillars of health: lose excess body fat (especially visceral fat), engage in regular strength training, prioritize 7-9 hours of sleep, manage stress, and eat a diet rich in protein, healthy fats, and zinc/magnesium.
7. Why is the test done in the morning?Testosterone follows a diurnal rhythm, peaking in the early morning (around 8 AM) and reaching its lowest point in the evening. Morning testing ensures measurement at the peak for accurate comparison to standard ranges.
8. Do women need testosterone?Absolutely. While needed in much smaller amounts, testosterone in women supports libido, bone density, muscle strength, and overall sense of well-being. Imbalances in either direction can cause significant symptoms.
9. Can low testosterone cause weight gain?Yes, it can create a vicious cycle. Low T can decrease muscle mass, slowing metabolism and promoting fat gain, particularly visceral fat. This fat tissue contains aromatase, which converts testosterone to estrogen, further lowering T levels.
10. At what age do male testosterone levels start to drop?Levels peak in early adulthood and then begin a gradual, steady decline of about 1% per year starting around age 30-40. However, a significant symptomatic drop is not a normal part of aging and should be evaluated.
Disclaimer: This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always consult your healthcare provider before making any changes to your health regimen or starting any new supplementation. Ready to understand your hormone health? Upload your lab results and get a personalized, AI-powered analysis of your testosterone and other key biomarkers. Sign up for HOP to analyze your labs with AI
